Transmission Electron Microscope Laboratory

About us

The Transmission Electron Microscope Laboratory offers imaging services for HUN-REN BRC researchers and for collaborators from other institutes. Using electron beam-based visualization, a resolution finer than 1 nanometer is making cell organelles and junctions, nanocarriers, viruses, vesicles, etc., visible.

Technology

Expertise and Equipment

JEOL JEM-1400Flash TEM

The 1400Flash is one of the latest models of the biological transmission electron microscopes of JEOL. With its upgraded Matataki Flash sCMOS camera, a resolution of 0.2 nanometers is achievable. Complementing the traditional transmission imaging, our microscope has a scanning transmission module and an x-ray detector, making chemical element analysis possible on certain samples. Additionally, the sample holder system is rotatable – with the help of an image array processing software package, construction of nano-scaled tomograms is possible.

Sample types

we have expertise in:
- Tissue samples: mammal and drosophila muscle and central nervous system samples, human muscle tissues, plant nodules and leaves, mushroom tissue
- Algae, bacteria, fungal spores, extracellular vesicles, bacteriophages, SARS-CO virus particle samples

Our best

Services

Sample fixation

Preparing Karnovsky fixative, sample fixation for electron microscopy (guidance if special fixation is needed)

Orientational sections

Semi-thin orientational section preparation for light microscopy

Sample preparation, staining

Sectioning embedded samples prepared for electron microscopy

Droplet sample preparation, staining

Preparing electron-transparent specimens (viruses, vesicles stc.)

Electronmicroscopy

Electron microscopy, aqcuisition and tomography

Post processing

Performing measurements on the images, figure preparation

Articles

Key publications

  • Koncz, M., Stirling, T., Hadj Mehdi, H., Méhi, O., Eszenyi, B., Asbóth, A., Apjok, G., Tóth, Á., Orosz, L., Vásárhelyi, B. M., Ari, E., Daruka, L., Polgár, T. F., Schneider, G., Zalokh, S. A., Számel, M., Fekete, G., Bohár, B., Nagy Varga, K., Visnyovszki, Á., … Kintses, B.:
    Genomic surveillance as a scalable framework for precision phage therapy against antibiotic-resistant pathogens.
    Cell, S0092-8674(24)01027-4
  • Farkas, D., Szikora, S., Jijumon, A. S., Polgár, T. F., Patai, R., Tóth, M. Á., Bugyi, B., Gajdos, T., Bíró, P., Novák, T., Erdélyi, M., & Mihály, J.:
    Peripheral thickening of the sarcomeres and pointed end elongation of the thin filaments are both promoted by SALS and its formin interaction partners.
    PLoS Genetics, 20(1), e1011117
  • Devadasu E., Kanna S.D., Neelam S., Yadav R.M., Nama S., Akhtar P., Polgár T.F., Ughy B., Garab G., Lambrev P.H., Subramanyam R.:
    Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase.
    Frontiers in Plant Science, 14:1051711
  • Mészáros M., Phan T.H.M., Vigh J.P., Porkoláb G., Kocsis A., Páli E.K., Polgár T.F., Walter F.R., Bolognin S., Schwamborn J.C., Jan J.S., Deli M.A., Veszelka S.:
    Targeting Human Endothelial Cells with Glutathione and Alanine Increases the Crossing of a Polypeptide Nanocarrier through a Blood-Brain Barrier Model and Entry to Human Brain Organoids.
    Cells, 12(3):503
  • Hudák A., Morgan G., Bacovsky J., Patai R., Polgár T.F., Letoha A., Pettko-Szandtner A., Vizler C., Szilák L., Letoha T.:
    Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice.
    International Journal of Molecular Sciences, 23(14):7609
  • Veszelka Sz., Mészáros M., Porkoláb G., Szecskó A., Kondor N., Ferenc Gy., Polgár T.F., Katona G., Kóta Z., Kelemen L., Páli T., Vigh J.P., Walter F.R., Bolognin S., Schwamborn J.C., Jan J., Deli M.A.:
    A Triple Combination of Targeting Ligands Increases the Penetration of Nanoparticles across a Blood-Brain Barrier Culture Model.
    Pharmaceutics, 14:86
  • Kartali T., Nyilasi I., Kocsubé S., Patai R., Polgár T.F., Zsindely N., Nagy G., Bodai L., Lipinszki Z., Vágvölgyi Cs., Papp T.:
    Characterization of Four Novel dsRNA Viruses Isolated from Mucor hiemalis Strains.
    Viruses, 13:2319
  • Koderi V. S., Shetty P., Deim Z., Terhes G., Urbán E., Váczi S., Patai R., Polgár T.F., Pertics B. Zs., Schneider Gy., Kovács T., Rákhely G.:
    Survival Comes at a Cost: A Coevolution of Phage and Its Host Leads to Phage Resistance and Antibiotic Sensitivity of Pseudomonas aeruginosa Multidrug Resistant Strains.
    Frontiers in Microbiology, 12:783722
  • Polgár T.F., Meszlényi V., Nógrádi B., Körmöczy L., Spisák K., Tripolszki K., Széll M., Obál I., Engelhardt J.I., Siklós L., Patai R.:
    Passive Transfer of Blood Sera from ALS Patients with Identified Mutations Results in Elevated Motoneuronal Calcium Level and Loss of Motor Neurons in the Spinal Cord of Mice.
    International Journal of Molecular Sciences, 22:9994
  • Topal G. R., Mészáros M., Porkoláb G., Szecskó A., Polgár T.F., Siklós L., Deli M. A., Veszelka Sz., Bozkir A.:
    ApoE-Targeting Increases the Transfer of Solid Lipid Nanoparticles with Donepezil Cargo across a Culture Model of the Blood–Brain Barrier.
    Pharmaceutics, 13(1):38
  • Papp A., Horváth T., Igaz N., Gopisetty M.K., Kiricsi M., Berkesi D.S., Kozma G., Kónya Z., Wilhelm I., Patai R., Polgár T.F., Bellák T., Tiszlavicz L., Razga Z., Vezér T.:
    Presence of Titanium and Toxic Effects Observed in Rat Lungs, Kidneys, and Central Nervous System in vivo and in Cultured Astrocytes in vitro on Exposure by Titanium Dioxide Nanorods.
    International Journal of Nanomedicine, 15:9939–9960
  • Meszlényi V., Patai R., Polgár T.F., Nógrádi B., Körmöczy L., Kristóf R., Spisák K., Tripolszki K., Széll M., Obál I., Engelhardt J.I., Siklós L.:
    Passive Transfer of Sera from ALS Patients with Identified Mutations Evokes an Increased Synaptic Vesicle Number and Elevation of Calcium Levels in Motor Axon Terminals, Similar to Sera from Sporadic Patients.
    International Journal of Molecular Sciences, 21:5566
  • Zsiros O., Nagy G., Patai R., Solymosi K., Gasser U., Polgár T.F., Garab G., Kovács L., Hörcsik Z.T.:
    Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis.
    Frontiers in Plant Science, 11:1006
  • Kartali T., Nyilasi I., Szabó B., Kocsubé S., Patai R., Polgár T.F., Nagy G., Vágvölgyi Cs., Papp T.:
    Detection and Molecular Characterization of Novel dsRNA Viruses Related to the Totiviridae Family in Umbelopsis ramanniana.
    Frontiers in Cellular and Infection Microbiology, 9:249

Team

Check our Team

Tamás Ferenc POLGÁR

head of laboratory

Erika BÁNFINÉ RÁCZ

laboratory assistant/administrator expert